Journal: PLoS ONE
Article Title: Antibody-Based Detection and Inhibition of Vaginolysin, the Gardnerella vaginalis Cytolysin
doi: 10.1371/journal.pone.0005207
Figure Lengend Snippet: (A) Human erythrocytes were exposed to varying concentrations of purified recombinant VLY for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
Article Snippet: Purified VLY toxin was generated and submitted to Cocalico Biologicals (Reamstown, PA).
Techniques: Purification, Recombinant, Lysis